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nf κbp65  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology nf κbp65
    Nf κbp65, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nf+%CE%BAbp65/NF%CE%BAB+p50/pmc13035809-316-6-20
    Average 95 stars, based on 461 article reviews
    nf κbp65 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: The inflammatory bowel disease and gut microbiome are restored by employing metformin-loaded alginate-shelled microcapsules.
    Article Snippet: Inflammatory bowel disease (IBD) is a chronic or recurrent inflammatory disorder affecting various parts of the gastrointestinal tract.. Metformin, a widely prescribed hypoglycemic drug for type 2 diabetes, has shown potential in reducing IBD risk.. However, its oral administration faces significant challenges due to the harsh gastrointestinal environment, requiring higher or more frequent doses to achieve therapeutic effects, which increases the risk of side effects.

    Western Blot:

    Article Title: Empagliflozin mitigates methotrexate-induced hepatotoxicity: Targeting ASK-1/JNK/Caspase-3 pathway.
    Article Snippet: Methotrexate (MTX) administration causes hepatotoxicity, a serious side effect limiting its clinical use.. Therefore, this study was performed to investigate the beneficial effect of empagliflozin (Empa) against MTX-induced hepatotoxicity.. Adult male albino mice were pre-treated with Empa (at 10 or 25 mg/kg/d, orally) for 6 days and then received a single MTX injection (at 20 mg/kg, intraperitoneally).

    Incubation:

    Article Title: Intermittent mild cold stimulation alleviates cold stress-induced pulmonary fibrosis by inhibiting the TGF-β1/Smad signaling pathway in broilers
    Article Snippet: The gels containing target proteins were transferred to nitrocellulose membranes using a trans-blot SD semidry electrophoretic transfer cell (Bio-Rad Laboratories, Hercules, CA) at 12 V for 35 min. .. Subsequently, the membranes were blocked with 5% skim milk for 1 h, and then incubated overnight at 4°C with the corresponding primary antibodies against HO-1 (WL02400, 1:1,500, Wanleibio), Nrf2 (WL02135, 1:500, Wanleibio), SOD2 (WL02506, 1:500, Wanleibio), TGF-β1 (WL02998, 1:1,000, Wanleibio), Smad2/3 (WL01520, 1:1,000, Wanleibio), phosphorylated Smad2/3 ( p-Smad2/3 , WL02305, 1:500, Wanleibio), Smad7 (WL02975, 1:500, Wanleibio), COL1 (14695-1-AP, 1:2,000, Proteintech), COL3 (22734-1-AP, 1:1,000, Proteintech), TIMP-2 (WL01209, 1:1,500, Wanleibio), NF-κBp65 (sc-8008, 1:300, Santa Cruz Biotechnology), IL-1β (WL00891, 1:500, Wanleibio), iNOS (22226-1-AP, 1:500, Proteintech), NLRP3 (WL02635, 1:1,500, Wanleibio), and β-actin (ab8227, 1:7,000, Abcam). .. After washing 3 times for 5 min each with TBST, the membranes were incubated with a secondary antibody of a goat anti-rabbit IgG labeled with horseradish peroxidase (1:10,000, Bioss Antibodies) for 1 h at room temperature.

    Article Title: Spinach Extract Reduces Kidney Damage in Diabetic Rats by Impairing the AGEs/RAGE Axis
    Article Snippet: The slides were mounted in a Shandon Sequenza chamber (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and incubated with blocking universal reagent (PolyVue ® mouse/rabbit DAB detection system, Diagnostic BioSystems, Pleasanton, CA, USA) at room temperature for one hour. .. Immediately, Primary antibody dilutions 1:100 for anti-CML (ab124145; Abcam PLC, Cambridge, UK), RAGE (sc-7269; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), NF-κBp65 (sc-8008; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), TNF-α (ab1793), IL-1β (sc-12742), casp-3 (sc-1224; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), SMAD2/3 (ab63399), TGF-β1 (sc-31609), α-SMA (sc-53142; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), CTGF (sc-365970; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), and NOX4 (ab133303, Abcam PLC, Cambridge, UK) were added and incubated overnight at 4 °C. ..



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